Vivien Walter is performing his PhD under the supervision of Carlos Marques at the Institut Charles Sadron (ICS) in Strasbourg, France. His work focus on the effect of the membrane composition in the interaction between arginine-rich cell-penetrating peptides and giant unilamellar vesicles.
He studied at the University of Strasbourg, where he obtained his BSc of Physics and Chemistry, then continued to his teaching’s MSc of Ph/Ch and achieved the « CAPES de Physique-Chimie », later pursued to his research’s MSc of Physics of Materials.
Weinberger, A., Walter, V., MacEwan, S. R., Schmatko, T., Muller, P., Schroder, A. P., et al. (2017). Cargo self-assembly rescues affinity of cell-penetrating peptides to lipid membranes. Sci Rep, 7, 43963.
Abstract: Although cationic cell-penetrating peptides (CPPs) are able to bind to cell membranes, thus promoting cell internalization by active pathways, attachment of cargo molecules to CPPs invariably reduces their cellular uptake. We show here that CPP binding to lipid bilayers, a simple model of the cell membrane, can be recovered by designing cargo molecules that self-assemble into spherical micelles and increase the local interfacial density of CPP on the surface of the cargo. Experiments performed on model giant unilamellar vesicles under a confocal laser scanning microscope show that a family of thermally responsive elastin-like polypeptides that exhibit temperature-triggered micellization can promote temperature triggered attachment of the micelles to membranes, thus rescuing by self-assembly the cargo-induced loss of the CPP affinity to bio-membranes.