Home > Systems & Techniques > Techniques > Optical Microscopies

Optical Microscopies

We use a wide range of optical microscopy techniques: Contrast Phase Microscopy, Fluorescence Microscopy, Confocal Microscopy, RICM...

  • phase separated GUVs Confocal Microscopy

    We use confocal microscopy to image the distribution of fluorescent molecules in the vesicle membrane.
    The microscope chassis is the inverted TE2000 Nikon. It is equipped with both traditional bright field, and epifluores- cence modules. The following objectives are available: x10, x20, x40 DIC, x40 Phase Contrast, x60 WI DIC, x100 OIL. The confocal module is available with the following laser wavelengths: 408 nm, 456-477-488-514 nm, and 543 nm. Images at three wavelengths can be acquired simultaneously, using three PMTs. Alternatively, images can be acquired in the ’spectral mode’, using a unique 32-channel PMT spectral detector, bandwidth per channel: 2.5, 5, or 10 nm.

  • Reflection Interference Contrast Microscopy (RICM)

    RICM = Reflexion Interference Contrast Microscopy.
    RICM is an interferometric technique suited to visualize objects in the vicinity of a flat substrate. It is very well adapted for membrane contour analysis above a glass coverslip.
    Reflection Interference Contrast Microscopy (RICM) is devoted to the observation of an object on a glass surface, with a normal-to-the slide optical penetration depth of typically 500 nm. Grey levels in the image reflect both the glass-object distance (potential sensitivity of 10 nm), and the refractive index variations along the object surface. The technique can be used for example to image cell spreading, or to quantify substrate-bilayer interactions.

  • Fluorescence Microscopy

Site powered by SPIP | | Site Map | Follow site activity RSS 2.0
Graphic design (c) digitalnature under License GPL