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Author (up) Micheletto, Y.M.S.; Moro, C.F.; Lopes, F.C.; Ligabue-Braun, R.; Martinelli, A.H.S.; Marques, C.M.; Schroder, A.P.; Carlini, C.R.; da Silveira, N.P. url  doi
  Title Interaction of jack bean (Canavalia ensiformis) urease and a derived peptide with lipid vesicles Type Journal Article
  Year 2016 Publication Colloids and Surfaces. B, Biointerfaces Abbreviated Journal Colloids Surf B Biointerfaces  
  Volume 145 Issue Pages 576-585  
  Keywords Jaburetox-2Ec; Jack bean urease; Light scattering; Liposomes; Saxs  
  Abstract Ureases are metalloenzymes that catalyze the hydrolysis of urea to ammonia and carbon dioxide. Jack bean (Canavalia ensiformis) produces three isoforms of urease (Canatoxin, JBU and JBURE-II). Canatoxin and JBU display several biological properties independent of their ureolytic activity, such as neurotoxicity, exocytosis-inducing and pro-inflammatory effects, blood platelets activation, insecticidal and antifungal activities. The Canatoxin entomotoxic activity is mostly due to an internal peptide, named pepcanatox, released upon the hydrolysis of the protein by insect cathepsin-like digestive enzymes. Based on pepcanatox sequence, Jaburetox-2Ec was produced in Escherichia coli. JBU and its peptides were shown to permeabilize membranes through an ion channel-based mechanism. Here we studied the JBU and Jaburetox-2Ec interaction with platelet-like multilamellar liposomes (PML) using Dynamic Light Scattering and Small Angle X-ray Scattering techniques. We also analyzed the interaction of JBU with giant unilamellar vesicles (GUVs) using Fluorescence Microscopy. The interaction of vesicles with JBU led to a slight reduction of hydrodynamic radius, and caused an increase in the lamellar repeat distance of PML, suggesting a membrane disordering effect. In contrast, Jaburetox-2Ec decreased the lamellar repeat distance of PML membranes, while also diminishing their hydrodynamic radius. Fluorescence microscopy showed that the interaction of GUVs with JBU caused membrane perturbation with formation of tethers. In conclusion, JBU can interact with PML, probably by inserting its Jaburetox “domain” into the PML external membrane. Additionally, the interaction of Jaburetox-2Ec affects the vesicle's internal bilayers and hence causes more drastic changes in the PML membrane organization in comparison with JBU.  
  Address Graduate Program in Chemistry, Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address:  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0927-7765 ISBN Medium mcube  
  Area Expedition Conference  
  Notes PMID:27281243 Approved no  
  Call Number ICS mcube @ thierry.charitat @ Serial 13986  
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