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Author (up) Micheletto, Y.M.S.; Marques, C.M.; Silveira, N.P. da; Schroder, A.P. url  doi
  Title Electroformation of Giant Unilamellar Vesicles: Investigating Vesicle Fusion versus Bulge Merging Type Journal Article
  Year 2016 Publication Langmuir Abbreviated Journal Langmuir  
  Volume 32 Issue 32 Pages 8123-8130  
  Abstract Partially ordered stacks of phospholipid bilayers on a flat substrate can be obtained by the evaporation of a spread droplet of phospholipid-in-chloroform solution. When exposed to an aqueous buffer, numerous micrometric buds populate the bilayers, grow in size over minutes, and eventually detach, forming the so-called liposomes or vesicles. While observation of vesicle growth from a hydrated lipid film under an optical microscope suggests numerous events of vesicle fusion, there is little experimental evidence for discriminating between merging of connected buds, i.e., a shape transformation that does not imply bilayer fusion and real membrane fusion. Here, we use electroformation to grow giant unilamellar vesicles (GUVs) from a stack of lipids in a buffer containing either (i) nanometric liposomes or (ii) previously prepared GUVs. By combining different fluorescent labels of the lipids in the substrate and in the solution, and by performing a fluorescence analysis of the resulting GUVs, we clearly demonstrate that merging of bulges is the essential pathway for vesicle growth in electroformation.  
  Address Institut Charles Sadron, Universite de Strasbourg, UPR22-CNRS , 23 rue du Loess, 67034 Strasbourg, France  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0743-7463 ISBN Medium mcube  
  Area Expedition Conference  
  Notes PMID:27409245 Approved no  
  Call Number ICS mcube @ thierry.charitat @ Serial 13990  
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